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Colloidal Coomassie Blue (Safe Stain)


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Description

Coomassie Blue (CCB) is widely used in visualising proteins separated by either agarose or acrylamide gel electrophoresis.

The use of which normally involves lengthy staining and de-staining of gels using both glacial acetic acid and methanol.  These solvents are both toxic and poisonous and need to be disposed of with hazardous waste.  In order to avoid these materials we have formulated coomassie blue G250 into a colloid which is both non-toxic and non hazardous.  The colloidal solution represents a safer way to apply the dye to the gels and is both easy and less expensive to use.

The CCB dye is applied to the gel after washing the gel with water, the CCB solution is then agitated until the bands start to appear whtin the gel which normally takes from 20 mins to 2 hours.  Once they appear and are visually strong enough, simply pour off the CCB stain and wash the gel in water.  Keep washing until any background staining is removed and the protein bands appear more intense.  If very small quantities of protein are being run, the staining can take place overnight and then extensively washed with water to visualise.



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