Molecular Biology Reagents

Severn Biotech supplies a wide range of Molecular Biology and Electrophoresis reagents. These include TEMED, APS, Boric acid, DTT, EDTA, Formamide, glycine, SDS Tris and Urea. These are all supplied to exacting standards to give the best end result possible.

Various pack sizes are available in the above and prices are competitive to supply the best quality product at the best possible outlay.

Agarose

Severn Biotech’s range of high-quality agaroses are all prepared to exacting standards which ensure that our products can be relied on time after time to produce optimal performance. By altering critical characteristics, the agaroses have been developed to meet the specific requirements of a diverse range of applications. The main characteristics are:

• Melting/Gelling Temperatures
• Gel Strength
• Electroendosmosis (EEO)

Gels with low melting temperatures (below the temperature at which DNA denatures) allow DNA to remain intact on melting, thus enabling easy purification of nucleic acid using B-agarase. SB Low Gel and Recovery will remain liquid for several hours at temperatures compatible with many in-gel enzymatic reactions and can be performed without having to separate the nucleic acids for the media. High gel strength is important if the application involves handling and mechanical manipulation such as Southern or Northern Blotting. Finally the EEO (the movement of non-charged molecules through a medium towards the cathode during electrophoresis) of an agarose will determine the mobility of certain macromolecules within the gel. Low EEO values allow nucleic acids to migrate faster thereby improving their resolution since migration is determined by size only.

The agaroses with Severn Biotech’s range can be used for a wide variety of applications from nucleic acid separation to immunoelectrophoresis and from tissue cell culture to viral plaque assays. Recommended applications are indicated with the description of each agarose type.

Ordering Information

Electrophoresis Consumables

Severn Biotech manufactures a range of liquid acrylamide pre-mix solutions for the production of Acrylamide Gels. These are exclusively used in Electrophoretic separation of DNA and Proteins.

Bespoke solutions

Please contact us for any product that is not listed as we can manufacture to order please quote your formula and catalogue number 20-XXXX-10  

[Ordering]

Design a Gel Acrylamide Solutions

The range includes a number of both Concentrates and Ready to use (RTU) Acrylamide Gel solutions.

By far the most ubiquitous product is 40% Acrylamide concentrate, which can be used with Bis-acrylamide to formulate a number of different solutions to suit the researchers needs.

The majority of Concentrates supplied are available with Bis in ratios 19:1 for DNA work, 37.5:1 for Protein separation through to higher ratios for specific molecular separation.

Ready to Use products are made with lower concentrations of Acrylamide i.e. 4-6% with typical Bis ratios of 19:1, with the inclusion of the denaturant Urea (6-8 Molar) and zwitterionic buffer at a concentration of 1x TBE.

Severn Biotech Ltd products are made to exacting standards and are tested for DNAase, RNase and Protease's, each lot is tested for performance to ensure there are no batch to batch variations. The variety of products are formulated to span the range of different techniques available to the researcher.

Acrylamide Solutions

Ready to Use Sequencing Gel Solutions

"Crystal Buffers"

Over the last 6 years Severn Biotech has successfully introduced a number of new buffer products which are specific to both Molecular and Cell biologists. The range is called Crystal Buffers and offers Crystal clear solutions to Molecular Biologists.

All products are molecular biology grade and tested for the absence of DNase, RNase and Proteases.

The pre-formulated Crystal Buffer range are designed for all your electrophoresis, blotting and hybridisation techniques.

The product range includes different Tris buffers such as Tris Acetate, Tris Borate, SSC, SSPE.

TBE Buffer

To accompany the range of sequencing products Tris borate EDTA running buffer is supplied to contain the same batch reagents which are used in Sequencing gel products. Each lot is tested for DNase, RNase and Protease and run tested on automated DNA sequencers.

Catalogue Numbers:-

Tris Borate EDTA buffer is made from Ultra pure molecular biology grade materials with a purity in excess of 99.8%. Bacterial filtered to remove the inclusion of nucleation centres, by the clarification of undissolved fine micro-crystallised material, TBE buffer is guaranteed to give perfect results.

Tris Glycine

The Crystal buffers have been made to exact formulations specified in various research papers or historically used to certain formulations which are already in widespread use.

i.e. Sambrook, Fritsch and Maniatis.

All buffer products are made from both molecular biology grade materials with ultrapure water and 0.2 micron filtered to ensure they are clarified and clear from nucleation points and tested for pH.

NB. Tris saline, Tris Tricine, Tris Taurine etc. are also available

Sterile Buffer solutions are now available as part of this range. One of the most popular sterile products is 1 X PBS Phosphate Buffered Saline which is used in numerous cell biology applications.

Phosphate Buffered Saline (1xPBS)

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