Material Safety Data Sheet Section 1. Chemical Product and Company Identification Common Name ThioLink Coupling Gel Supplier Severn Biotech Ltd Unit 2 Park Lane Kidderminster Worc's DY11 6TJ Manufacturer As above Code AB100 In Case of Emergency contact Severn Biotech Ltd Section 2. Composition and Information on Hazardous (OSHA) Ingredients Glycerol CAS # 56-81-5 % by Weight 35-50 Exposure Limits TWA: 10 (mg/m3) from ACGIH (TLV) [United States] TWA: 10 (ppb) from ACGIH (TLV) [United States] [1994] Inhalation TWA: 5 (mg/m3) from OSHA (PEL) [United States] [1989] Inhalation Respirable. Section 3. Hazards Identification Emergency Overview WARNING! BIRTH DEFECT HAZARD CONTAINS MATERIAL WHICH CAN CAUSE BIRTH DEFECT. CAUSES SEVERE RESPIRATORY TRACT AND SKIN IRRITATION. CONTAINS MATERIAL WHICH CAUSES DAMAGE TO THE FOLLOWING ORGANS: KIDNEYS, REPRODUCTIVE SYSTEM, MUCOUS MEMBRANES, GASTROINTESTINAL TRACT, RESPIRATORY TRACT, SKIN, EYES, EYE, LENS OR CORNEA. MAY CAUSE EYE IRRITATION. Avoid contact with eyes, skin and clothing. Avoid breathing vapor or mist. Keep container closed. Use only with adequate ventilation. Avoid exposure during pregnancy. Wash thoroughly after handling. Routes of Entry: Absorbed through skin. Eye contact. Inhalation. Ingestion. Potential Acute Health: Very hazardous in case of skin contact (irritant), of inhalation (lung irritant). Effects Hazardous in case of eye contact (irritant). Slightly hazardous in case of ingestion, Carcinogenic Effects Data CARCINOGENIC EFFECTS: Not available. MUTAGENIC EFFECTS: Not available. TERATOGENIC EFFECTS: Not available. Medical Conditions Repeated or prolonged exposure is not known to aggravate medical condition. Aggravated by Overexposure: EU Regulations R36/37/38- Irritating to eyes, respiratory system and skin. S2- Keep out of the reach of children. S46- If swallowed, seek medical advice immediately and show this container or label. See Toxicological Information (section 11) Section 4. First Aid Measures SKIN: Wash contaminated skin with soap and water. EYES: Flush with plenty of water for at least 15 minutes, occasionally lifting the upper and lower eyelids.Seek medical attention. Inhalation: Move exposed person to fresh air. If irritation persists, get medical attention. Ingestion: Do not induce vomiting. If affected person is conscious, give plenty of water to drink. Seek medical attention. Section 5. Fire Fighting Measures Flammability of Non-flammable. the Product Flash Points Not applicable. Fire Hazards in Presence Not applicable. Of Various Subs Fire Fighting Media Not applicable. and Instructions Protective Clothing (Fire) Not applicable. Section 6. Accidental Release Measures Small Spill and Leak Dilute with water and mop up, or absorb with an inert dry material and place in an appropriate waste disposal container. Finish cleaning by spreading water on the contaminated surface and dispose of according to local and regional authority requirements. Large Spill and Leak Absorb with an inert material and put the spilled material in an appropriate waste disposal. Finish cleaning by spreading water on the contaminated surface and allow to evacuate through the sanitary system. Be careful that the product is not present at a concentration level above TLV. Check TLV on the MSDS and with local authorities. Section 7. Handling and Storage Handling Avoid contact with eyes, skin, and clothing. Avoid breathing vapors or spray mists. Keep container closed. Use only with adequate ventilation. Wash thoroughly after handling. Storage Keep container tightly closed. Keep container in a cool, well-ventilated area. Store between 2 to 8°C (35.6 to 46.4°F). Section 8. Exposure Controls/Personal Protection Engineering Controls Provide exhaust ventilation or other engineering controls to keep the airborne concentrations of vapors below their respective threshold limit value. Personal Protection Eyes Splash goggles. Body Lab coat. Respiratory Vapor respirator. Be sure to use an approved/certified respirator or equivalent. Wear appropriate respirator when ventilation is inadequate. Hands Gloves. Section 9. Physical and Chemical Properties Boiling/Condensation Point The lowest known value is 290.05°C (554.1°F) (Glycerol). Melting/Freezing Point May start to solidify at 19.9°C (67.8°F) based on data for: Glycerol. Physical State and Liquid. (Suspension.) Appearance Molecular Weight Not applicable. Specific Gravity The only known value is 1.261 (Water = 1) (Glycerol). Vapor Pressure The highest known value is 3.17 (Air = 1) (Glycerol). Vapor Density The highest known value is <0.1 kPa (@ 20°C) (Glycerol). Dispersion Properties See solubility in water. Solubility Easily soluble in cold water. Partially soluble in hot water. Odor Not available. Taste Not available. Color Not available. Section 10. Stability and Reactivity Stability and Reactivity The product is stable. Incompatibility with Reactive with oxidizing agents, moisture. Various Substances Hazardous Polymerization Will not occur. Conditions of Instability Reacts violently with water especially when water is added to the product. Heating may cause an explosion. Keep away from heat (Sodium Azide) Hazardous Decomposition Not available. Products Section 11. Toxicological Information Toxicity to Animals Acute oral toxicity (LD50): 4090 mg/kg [mouse]. (Glycerol). Chronic Effects on DEVELOPMENTAL TOXICITY: Classified Reproductive system/toxin/female, Reproductive Humans system/toxin/male [PROVEN] [Glycerol]. Other Toxic Effects Hazardous in case of eye contact (irritant). Slightly hazardous in case of ingestion, . on Humans Special Remarks on Other To the best of our knowledge, the toxicological properties of this product have not been thoroughly Toxic Effects on Humans investigated. Section 12. Ecological Information Toxicity of the Products of The product itself and its products of degradation are not toxic. Biodegradation Section 13. Disposal Considerations Waste Information Waste must be disposed of in accordance with federal, state and local environmental control regulations. Waste Stream Not available. Section 14. Transport Information CONTACT SEVERN BIOTECH FOR TRANSPORTATION INFORMATION. Section 15. Regulatory Information HCS Classification CLASS: Target organ effects. CLASS: Reproductive toxins. U.S. Federal Regulations TSCA 8(b) inventory: Agarose; Ethylenediamine Tetraacetic Acid, Disodium Salt; Sodium Azide; Glycerol TSCA 8(d) H and S data reporting: Sodium Azide; Glycerol SARA 302/304/311/312 extremely hazardous substances: Sodium Azide SARA 302/304 emergency planning and notification: Sodium Azide SARA 302/304/311/312 hazardous chemicals: Sodium Azide; Glycerol SARA 311/312 MSDS distribution - chemical inventory - hazard identification: Sodium Azide: immediate health hazard; Glycerol: immediate health hazard, delayed health hazard Clean Water Act (CWA) 307: No products were found. Clean Water Act (CWA) 311: No products were found. Clean air act (CAA) 112 accidental release prevention: No products were found. Clean air act (CAA) 112 regulated flammable substances: No products were found. Clean air act (CAA) 112 regulated toxic substances: No products were found. WHMIS (Canada) CLASS D-2A: Material causing other toxic effects (VERY TOXIC). CEPA DSL: Agarose; Sodium Azide; Glycerol CEPA NDSL: Sodium Azide International Regulations Not available. EINECS Section 16. Other Information THE ABOVE INFORMATION IS BELIEVED TO BE CORRECT BUT DOES NOT PURPORT TO BE ALL INCLUSIVE AND SHALL BE USED ONLY AS A GUIDE. SEVERN BIOTECH SHALL NOT BE HELD LIABLE FOR ANY DAMAGE RESULTING FROM HANDLING OR FROM CONTACT WITH THE ABOVE PRODUCT.